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Scientists integrate single-cell processing and RT-qPCR for parallel analysis of hundreds of cells

from Genetic Engineering & Biotechnology News

Researchers report on the development of a fully integrated microfluidic device they claim can perform RT-qPCR measurements of gene expression in hundreds of single cells, in a single run. Uniquely, the 9V battery-sized device, developed by a team at the University of British Columbia, and the Center for Translational and Applied Genomics in Vancouver, Canada, has been designed to carry out all steps associated with single cell processing, including cell capture, cell lysis, reverse transcription and quantitative PCR. more


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