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Brightfield RNA in situ hybridization with RNAScope is one of the presentations featured at the July 18 Forum on Immunohistochemistry

from NSH

In situ hybridization is used by researchers to examine gene expression in the context of pathologic changes in tissues. This technique detects nucleic acids that are preserved in a tissue specimen by using hybridization probes that are complementary to the nucleic acid target and visualized with either fluorescent (FISH) or chromogenic (CISH) dyes. The general principles and technological advances for RNA ISH will be reviewed. Join us in Tennessee for the eighth annual IHC Forum where the application of the RNAScope method for FFPE tissues in research studies is discussed to demonstrate the capabilities of this more sensitive and specific technique for bright-field analyses of cytoplasmic mRNAs with several practical examples provided. This improved methodology relies on multiple targeted probes that are used to increase specific binding to genes of interest and for serial amplification of a signal. Learn more. more

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