Immuno-oncology is one of the hottest fields of research today, with a key focus to understand the tumor microenvironment composition and immune function. Infiltration of tumors is an important step in the host response to cancer. Identification, characterization, and quantification of these infiltrating immune cells have been limited by the small number of proteins that can be examined simultaneously within a single tissue section using standard colorimetric immunohistochemistry (IHC). The combination of multiple fluorophore-labeled antibodies overcomes this limitation and allows for simultaneous visualization of both classical cancer markers as well as a variety of immune cell antigens.
Generate clean and top-notch data. eBioscience® has the optimized reagents, proven protocols, and the technical expertise to help you with your IHC/ICC experiments from start to a successful finish. The images above reveal the identification of T and B cell populations in the tumor microenvironment. Formalin-fixed paraffin embedded (FFPE) human breast cancer tissue (infiltrating ductile carcinoma (IC) or ductile carcinoma in situ (DCIS)) was stained with three different eBioscience antibodies. Numerous CD45RB-positive cells (NK, myeloid, T, and B cells) localize in clusters adjacent to duct regions. CD45RB-positive cells (red) are interspersed among pan-cytokeratin-positive (green) and HER-4 (ErbB4)-positive cells (orange). Nuclei are blue.