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Pertussis diagnosis: Avoid the pitfalls of PCR

from Medscape

Polymerase chain reaction (PCR) is an important tool for diagnosing pertussis. PCR is a rapid test that detects DNA sequences of the Bordetella pertussis bacterium, and unlike culture, does not require viable bacteria. Despite these advantages, PCR can give inaccurate results. Many common pitfalls of PCR can be avoided by following best practices for who to test, when to test, collection techniques, and preventing contamination. more

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