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High-throughput screening for RNA interference

from By Dr. Afsaneh Motamed-Khorasani

RNA interference, or RNAi, involves the knockdown of specific gene functions. It is a reverse genetic approach for the functional analysis of a large number of genes. Furthermore, it offers the identification of structure or function of the genes, relevant to specific pathway by gene knockdown mechanisms. RNAi screening is also used for better understanding of host-pathogen interaction and cancer biology. RNAi screening has also been automated for high-throughput drug discovery studies and requires a good knowledge of computer science and engineering for automated high-content image acquisition and analysis. more

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