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Contamination control using hot air sterilization in carbon dioxide incubators


Microbial contamination poses a major risk in cell culturing and remains undetected for a long time since contamination does not necessarily overgrow the cultivated cells. Subtle changes such as lack of essential nutrients and excretion of microbial metabolites may promote a pH change which ultimately compromises cell proliferation. Mycoplasma infections pose the highest risk and can change host cell morphology or cause chromosomal aberrations. This article compares different contamination control concepts for CO2 incubators from the user's perspective. more

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